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ligation efficiency

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  • Sequencing by ligation — is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike most currently popular DNA sequencing methods, this method does not use a DNA polymerase to create a… …   Wikipedia

  • DNA ligase — repairing chromosomal damage Identifiers EC number 6.5.1.1 …   Wikipedia

  • Chemical biology — is a scientific discipline spanning the fields of chemistry and biology that involves the application of chemical techniques and tools, often compounds produced through synthetic chemistry, to the study and manipulation of biological systems.… …   Wikipedia

  • ChIA-PET — Chromatin Interaction Analysis by Paired End Tag Sequencing (ChIA PET) is a technique that incorporates chromatin immunoprecipitation (ChIP) based enrichment, chromatin proximity ligation, Paired End Tags, and ultra high throughput sequencing to… …   Wikipedia

  • Molecular Inversion Probe — (MIP)[1] belongs to the class of Capture by Circularization molecular techniques [1] for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome[2]. Probes used in this technique are… …   Wikipedia

  • Chromosome conformation capture — Technologies Chromosome conformation capture,[1] or 3C, is a high throughput molecular biology technique used to analyze the organization of chromosomes in a cell s natural state. Studying the structural properties …   Wikipedia

  • Polymerase chain reaction — PCR redirects here. For other uses, see PCR (disambiguation). A strip of eight PCR tubes, each containing a 100 μl reaction mixture The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a… …   Wikipedia

  • Directionality (molecular biology) — A diagram of a furanose (sugar ring) molecule with carbon atoms labeled using standard notation Directionality, in molecular biology and biochemistry, is the end to end chemical orientation of a single strand of nucleic acid. The chemical… …   Wikipedia

  • MRNA display — is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a… …   Wikipedia

  • Variants of PCR — This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process. The versatility of PCR has led to a large number of variants: Contents 1 Basic modifications 2 Pretreatments and extensions 3 Other… …   Wikipedia

  • Transformation (genetics) — An unrelated process called malignant transformation occurs in the progression of cancer. In molecular biology transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation and expression of exogenous genetic… …   Wikipedia

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